Certified antibodies database
The NGS-QC team has setup a certification procedure for Antibodies dedicated to ChIP-seq applications based on:
- a highly reproducible pipeline for performing ChIP-seq assays including the use of an automated liquid handling platform (TECAN EVO75) for ChIP and sequencing library preparation steps.
- the use of the NGS-QC Generator tool for providing a ChIP-seq quality grade and to evaluate other parameters like the Optimal sequencing depth, the degree of reproducibility among biological replicates and the comparison of the quality grades of the certified antibody with datasets retrieved in the public domain.
This quality certification structure makes possible to assign a measured Quality Stamp to ChIP-seq grade Antibodies!
Antibody ID | Vendor | Antibody Type | Target Molecule | Antibody Batch | Certification Conditions | Global QC indicators | Sequencing Platform | Certification Date | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Organism | Cell Line | Replicate | TMRs | QC Stamps | QC report | 2.5pc denQC s50 | 2.5pc simQC s50/s90 | 5pc denQC s50 | 5pc simQC s50/s90 | 10pc denQC s50 | 10pc simQC s50/s90 | |||||||
39685 | Active Motif | monoclonal | H3K27ac | 16611003 | Homo sapiens (hg19) | Hela | 1 | 54058102 | AAB |
![]() | 0.434 | 8.820 | 1.755 | 3.650 | 5.002 | 1.737 | Illumina HiSeq 2500 | 2014-09-25 |
2 | 47113024 | ABB | 0.374 | 8.686 | 1.583 | 3.855 | 4.601 | 1.768 | Illumina HiSeq 2500 | 2014-09-25 | ||||||||
39133 | Active Motif | polyclonal | H3K27ac | 01613007 | Homo sapiens (hg19) | Hela | 1 | 24890602 | AAA |
![]() | 0.785 | 8.398 | 2.981 | 3.754 | 7.542 | 1.941 | Illumina HiSeq 2500 | 2014-09-25 |
2 | 46589190 | AAB | 0.574 | 5.390 | 1.836 | 2.889 | 4.278 | 1.585 | Illumina HiSeq 2500 | 2014-09-25 | ||||||||
61251 | Active Motif | monoclonal | H3K9ac | 04812001 | Homo sapiens (hg19) | Hela | 1 | 35103551 | BBB |
![]() | 0.165 | 24.167 | 1.302 | 7.598 | 5.206 | 2.523 | Illumina HiSeq 2500 | 2014-09-25 |
2 | 106875269 | AAA | 0.823 | 9.440 | 3.955 | 3.675 | 12.411 | 1.538 | Illumina HiSeq 2500 | 2014-09-25 | ||||||||
61379 | Active Motif | monoclonal | H3K4me3 | 35312001 | Homo sapiens (hg19) | Hela | 1 | 23663884 | BBC |
![]() | 0.209 | 12.762 | 0.783 | 4.722 | 1.864 | 2.483 | Illumina HiSeq 2500 | 2014-09-25 |
2 | 101679298 | AAA | 1.413 | 10.160 | 7.265 | 3.665 | 23.145 | 1.503 | Illumina HiSeq 2500 | 2014-09-25 | ||||||||
39159 | Active Motif | polyclonal | H3K4me3 | 12613005 | Homo sapiens (hg19) | Hela | 1 | 35450487 | ABB |
![]() | 0.395 | 3.970 | 1.083 | 2.519 | 1.974 | 1.653 | Illumina HiSeq 2500 | 2014-09-25 |
2 | 101671832 | AAB | 0.810 | 2.126 | 1.434 | 1.633 | 2.203 | 1.218 | Illumina HiSeq 2500 | 2014-09-25 | ||||||||
39917 | Active Motif | polyclonal | H3K9ac | 23012003 | Homo sapiens (hg19) | Hela | 1 | 37492305 | AAB |
![]() | 0.500 | 4.185 | 1.450 | 2.987 | 3.037 | 1.719 | Illumina HiSeq 2500 | 2014-09-25 |
2 | 35405394 | ABB | 0.358 | 5.952 | 1.224 | 3.374 | 2.790 | 1.853 | Illumina HiSeq 2500 | 2014-09-25 |